This essay Amylase, Catalase and Invertase Enzyme Labs has a total of 1267 words and 8 pages.
Amylase, Catalase and Invertase Enzyme Labs
IB Biology SL Y1
22 April 2014
Amylase, Catalase and Invertase Enzyme Labs
Catalase is responsible for converting hydrogen peroxide1, which is harmful within living organisms, into water and oxygen molecules. This experiment investigates the effect of hydrogen peroxide on boiled and raw materials such as potatoes, liver, yeast cells, etc. If boiled materials were put into hydrogen peroxide, there would be no significant enzymatic reaction because the boiling temperature would already denature catalase in those materials, preventing any enzymes from functioning properly.
Sucrose is hydrolyzed into monosaccharide form of fructose and glucose by invertase that catalyzes the hydrolysis1. Invertase can be obtained from yeast, which will be used in this experiment. The yeast suspension solution will show positive result to Benedict’s solution test that indicates the presence of sugar.
Amylase is an enzyme that breaks down starch into glucose through the process of hydrolysis2. It initiates the breakdown of starch to glucose in seeds during germination. To identify the presence of starch, iodine test will be used. If the result shows no color change into deep purple, that indicates the absence of starch and implies the presence of glucose that is broken down from starch. Boiled corn seeds would show least amount of color change in agar plate (from dark purple into transparent) because the high temperature would have already denatured amylase in seeds.
Aim of experiment
These three enzyme experiments aim to investigate each enzyme’s role in breaking macromolecules into simple molecules of smaller units.
Table 1.0 – Qualitative observation of catalase lab
Material/extract being tested Boiled extract’s reaction on H2O2 Raw extract’s reaction on H¬¬¬2O2
(No apparent reaction occurring) Solution quickly fluffed up with fine bubbles; the lower section of solution that was not fluffed was relatively transparent.
Corn leaf Subtle reaction of tiny bubbles slowly rose; solution remained dominantly clear.
Ground meat Solution reacted and created fine, creamy bubbles while lower part remained clear.
Yeast Solution was dominantly clear with tiny bubbles rising rapidly from the bottom.
Potato Subtle reaction of tiny bubbles slowly rose; solution remained dominantly clear.
Table 2.0 – qualitative observation of invertase lab
Sucrose solution being tested Glucose strip test Benedict’s solution
Yeast suspension Light green spots of 100mg/L Light yellow orangish solution that is translucent and milky
Distilled water Light green shades of 100mg/L but are spread out in a smoother manner Negative result: dark greenish brown color
Table 3.0 – qualitative observation of amylase lab
Types of corn seeds Reaction after applying iodine on agar plates
Soaked seeds Plates contained spots of transparent area where soaked corn seeds were place. Overall, there were tiny dots and large patches of dark blackish purple color on agar plate.
Boiled seeds No large patches of dark color, except similar tiny dots that were all over the agar’s surface. There were transparent spots where seeds were placed.
Dry seeds Traces of dark blackish purple color surrounded the areas where corn seeds were placed (transparent). Agar plate was filled with tiny black dots. Agar plate had the darkest shade of color compared to the rest.
The extracts that were experimented to investigate the enzymatic reaction of catalase on hydrogen peroxide (H2O2) included liver, corn leaf, ground meat, yeast, and potato. All the boiled extracts of these materials yielded no apparent enzymatic reaction when tested with H2O2. This proves how temperature, which is one of the factors that can impact enzymatic reaction, is responsible for chemical reactions not happening. Catalase in these materials were boiled at 100℃, apparently it exceeded each of their optimal temperature. Consequently, catalase within each material was denatured and not able to perform its function properly when tested with H2O2. On the other hand, all the raw extracts reacted to H2O2 at different degrees. Catalase in liver and ground meat extracts caused apparent reactions to H2O2 while catalase in corn lead, yeast, and potato extracts created less apparent enzymatic reaction to H2O2. Their differences in amount of reaction might be explained by various factors such as how pH level in raw meat and liver may be closer to the optimal pH level for catalase to catalyze.
This lab involves observing effects of adding yeast and distilled water to sucrose solution, which is an example of disaccharide. Despite the poor ability of glucose strips to indicate glucose’s presence, the use of Benedict’s solution allowed more reliable proof of glucose’s presence to be obtained. When added 10 drops of Benedict’s
Topics Related to Amylase, Catalase and Invertase Enzyme Labs
Essays Related to Amylase, Catalase and Invertase Enzyme Labs
Abuse Of The InnocentAbuse of the Innocent Is it right to force a mouse to live it's live in a laboratory cage to test anti-cancer drug? How would you like to be squeezed in a cage with many other animals, not being able to touch the grass, run around and play, smell the flowers, or go for a walk in the warmth of the sunshine? Animal cruelty is wrong because we are hurting the Innocent. Animals experience and feel pain, fear , anxiety, stress, depression, boredom, joy and happiness. Animals are very intelligent, som
The Forter Five Force of MCDThe Forter Five Force of MCD The Porter Five Forces The fast food industry is a revolutionary industry, which has taken the world by storm. With fast food restaurants showing up, what seems like on every corner, it?s interesting to analyze the competitive strategies companies in this industry use in order to survive. McDonald?s, with more than 30,000 restaurants worldwide is certainly no exception. A good way to analyze the strategies is by using the Porter five forces competitive model. The Por
The MongolsThe Mongols It has been said that the Mongols were the most cruel and barbaric of the peoples that have roamed this earth. My research paper is on the greatest of the Mongols, Genghis Khan. Genghis Khan was, even in the lightest sense, a military genius. Genghis Khan almost conquered the world. He instilled in humankind a fear that lasted for ages. But what drove him to do it? Was it by chance? This paper will explain how the general's childhood molded the man into the best war general of the kn
The Enviromnetal Degradation As A Result Of OverpopulationThe Enviromnetal Degradation as a Result of Overpopulation 1 Introduction There are simply too many people on our planet, and the population is not showing any signs of slowing down(see Figure 1). It is having disastrous effects on our environment. There are too many implications and interrelationships to discuss in this paper, but the three substances that our earth consists of: land, water and air, are being destroyed. Our forests are being cut down at an alarming rate, bearing enormous impact
PersonalPersonal Today there are thousands of terminally ill patients in long term care facilities across America. In care facilities there are a countless number of elderly people and terminally ill patients that have repeatedly expressed a desire to die. Should these people be allowed to die, or should they be forced to keep on living? This question has plagued ethicists and physicians for decades. Euthanasia is the act of killing or permitting the death of hopelessly sick or injured individuals in a
EthologyEthology The biological study of animal behavior is called Ethology. All behavior is a reaction to a stimulus. John B. Watson influenced animal behavior in the twentieth century. He published a book in 1924 called Behaviorism. Jakob von Uexkull and O. Heinroth started a school that taught about animal behavior. There are two categories of animal behavior: genetically determined or environmentally determined. Animal behavior is the different ways that animals do different things, such as hunt
Which Of Your Possessions Reveals Most About You And Why?Which of your possessions reveals most about you and why? University of Washington Observe, Watson. An unfamiliar voice caused me to stop dead in my tracks as I was coming back to my table. Surreptitiously glancing through the foliage, I noticed two men pointing at something. Hesitant to announce my presence, I was very perplexed by the fact that the object they were staring at was my backpack! I was studying in the park and walked off to get a drink of water, and when I came back, whom should
Reflections On The Things They CarriedReflections on The Things They Carried Part I: Analyzing the story?s craftsmanship Tim O?Brien wrote a story that is known as The Things They Carried. It is a carefully crafted, detailed account of a Lieutenant and his men, the time period being right in the middle of the Vietnam war. In most war stories the author spends most of his or her time describing actions and events to the reader, trying to really put the reader right there in the middle of everything that is happening. However, O
To What Extent Does Acid Precipitation Affect Annelids?To What Extent Does Acid Precipitation Affect Annelids? In order to truly understand acid rain and it's eventual effect on earthworms, it would be best to look at the causes of acid rain. How and why does altered acidity in precipitation have a devastating effect? Acid rain is charecterized as Precipitation that has a pH lower than about 5.0 (Allaby, Michael (1994) Ecology, Oxford Press,). Acid rain is created by many things, of which pollution from cars contributes the most. Ever since the In
NitrogenNitrogen Nitrogen, symbol N, gaseous element that makes up the largest portion of the earth's atmosphere. The atomic number of nitrogen is 7. Nitrogen is in group 15 (or Va) of the periodic table. Nitrogen was discovered by the British physician Daniel Rutherford in 1772 and recognized as an elemental gas by the French chemist, Antoine Laurent Lavoiser about 1776. Nitrogen is a colorless, odorless tasteless, nontoxic gas. It can be condensed into a colorless liquid, which can be compressed into
SHOULD CONGRESS DOWNSIZE THE U.S. MILITARY BUDGET?SHOULD CONGRESS DOWNSIZE THE U.S. MILITARY BUDGET? The issue of defense of the countru should be priority for every government. It is very good that American government created such strong army with the most modern machines and system of defense. The United States should be aware of conflicts and terrorists' acts. It costs a lot of money, but safety of citizens should be provided in the first place. Every American feels, that he is his own country and he can be sure that in case of the war he
OUR FOOD SYSTEMOUR FOOD SYSTEM Group1 1 After a long hard day of work you sit down in your comfortable recliner and open up your favorite snack. But when you reach into grab a piece, you pull out a dead bug. Suddenly many thoughts come into your mind, you wonder how did the bug get there and was it dead or alive. Is it harmful or carry a disease. You ask yourself did the bug come from the United States or another country and where was your snack made? As all these questions come into your head, you wonder who
Respiratory SystemRespiratory System Respiratory System The main organs of the respiratory system are the lungs, trachea, bronchioles, mouth, nose and epiglottis. The accessory organs are the bladder, lungs, large intestine, kidney and pancreases. Anatomy and Physiology of the Respiratory System The respitory system tract is divided into the upper and lower regions. The upper respoitory tract includes the nasal passages, sinuses, and the throat area where the epiglottis and larynx are located. The lungs are a pai
Digestion and AbsorptionIntroduction The key issue in the digestion and absorption of fats is one of solubility. Lipids are hydrophobic, and thus are poorly soluble in the aqueous environment of the digestive tract. The digestive enzyme, lipase, is water soluble and can only work at the surface of fat globules. Digestion is greatly aided by emulsification, the breaking up of fat globules into much smaller emulsion droplets. Bile salts and phospholipids are amphipathic molecules that are present in the bile. Motility in
EMULSIFICATION AND DIGESTION OF LIPIDS BY BILE AND LIPASENAME : CLASS : DBT 4A DATE SUBMITTED : 14 AUG 2015 EXPERIMENT 3: EMULSIFICATION AND DIGESTION OF LIPIDS BY BILE AND LIPASE (VIRTUAL) INTRODUCTION Lipase is a type of enzyme known as a hydrolase and is responsible for catalysing the hydrolysis of triglycerides (the substrate) into fatty acids and glycerol. It is referred to as a hydrolase because the reaction that it catalyses is a hydrolysis reaction in which large molecules are broken down into smaller ones with the addition of water. Lipase is
Dialysis Dialysis Biology 202A Anatomy & Physiology II Professor Debra Dutton September 27, 2012 Throughout the United States and other countries, there are many people that suffer from renal failure every day. Dialysis is a way of helping with the kidneys. The kidneys are responsible for filtering all of the wastes products from the blood. It restores the proper balance of electrolytes in the body and eliminates extra fluid from the body also. Even though many individual’s kidneys don’t work as properl
IB Biology syllabus – definitionsIB Biology syllabus – definitions * Definition given in IB Biology syllabus TOPIC WORD DEFINITION Topic 1: Cells Organelle A discrete structure within a cell, with a specific function.* Tissue A group of cells similar to each other, along with their associated intercellular substances, which perform the same function within a multicellular organism. Organ A group of tissues which work together as a single unit to perform a particular function within a multicellular organism. Organ system A group
Anthropology is a discipline that systematically Anthropology is a discipline that systematically studies human culture and the evolutionary dimensions of human biology. It let us help to get information about humans with their past and present. Anthropology relates to other dimensions of studies such as biological science and physical science to get the understanding of human history and cultures. It is also a study of the biological human with their physical elements. Anthropology is studied under four main subfields; Cultural Anthropolog
History of Benjamin Banneker High School Howard University College of Dentistry Dental Hygiene Department School Based Program Benjamin Banneker Academic High School Nicole Johnson 82409-406-01/Dental Health Education Methods Professor Dawn Smith 09/06/2015 School Based Program History of Benjamin Banneker High School Originally, Benjamin Banneker, in August 1981, opened its doors as an alternative public institution of secondary education. Named after the districts’ renowned surveyor on the six-man team, which helped design, the blue
History of Benjamin Banneker High School Howard University College of Dentistry Dental Hygiene Department School Based Program Benjamin Banneker Academic High School Nicole Johnson 82409-406-01/Dental Health Education Methods Professor Dawn Smith 09/06/2015 School Based Program History of Benjamin Banneker High School Originally, Benjamin Banneker, in August 1981, opened its doors as an alternative public institution of secondary education. Named after the districts\' renowned surveyor on the six-man team, which helped design, the blu
A danger to public health and welfareA danger to public health and welfare In what could be a historic moment in the struggle against climate change, the environmental protection agency on Friday confirmed what most people have long suspected but had never been declared as a matter of federal law’s carbon dioxide and other greenhouse gases constitute a danger to public health and welfare. The formal “endangerment finding” names carbon dioxide and five other heat trapping gases as pollutants subject to regulation under the federal c
Global warming Aim To investigate how humans are contributing to climate change, the effect of this on social, environmental and political situations around the world and how organisations are trying to better manage the situation. Global warming Global warming is the term used to describe a gradual increase in the average temperature of the Earth’s atmosphere and its oceans, a change that is believed to be permanently changing the Earth’s climate. Even though it is an ongoing debate, it is proved by the scie
Life after Death - notesLife after Death - notes Pascal\'s Wager is the idea that, if you don\'t know whether there is an afterlife. Dualists • There are two aspects to human beings, a physical body and a non-physical soul • They tend to believe in life after death Monists/materialists The monist view states that we are physical bodies only. Once we die, that\'s it. Emotions are simply psycho-chemical reactions and nothing more. The Problem Whether the mind and body are one of the same nature (monistic view) or whether
LipaseBackground information: Enzymes are biological molecules (proteins) that act as catalysts and help complex reactions occur. The enzyme grabs on to the substrate at a special area called the active site. The combination is called the enzyme/substrate complex. Enzymes are very specific and don\'t just grab on to any molecule. The active site is a specially shaped area of the enzyme that fits around the substrate. The activity of enzymes is affected by three factors; temperature, concentration, and
Infancy: A Solid StartInfancy: A Solid Start • Birth-3 years old Physical Growth 1. Nervous System a. Brain growth b. Basic unit of neuronal communication is neurons c. Plasticity – Can grow or lose synaptic connections d. Sleep 16-17 hours in a 24-hour period • At 4 months can sleep through night e. Baby fitful, restless, breathing/heart rate/blood pressure increase in sleep f. SIDS is leading cause of death during infancy, baby just stops breathing 2. Motor Development a. Babies born with reflexes for survival • Ro
MASTER OF BUSINESS ADMINISTRATION MASTER OF BUSINESS ADMINISTRATION ADVANCED CORPORATE STRATEGY ( MGT 6266 ) INDIVIDUAL COMPANY CASE STUDY Dutch Lady Milk Industries Berhad Report prepared by: Abdulla Abdulqader Al-Attas (G1430873) Contents TOC \o 1-3 \h \z \u Introduction PAGEREF _Toc467903143 \h 3 Mission, Visions, Value, and Objectives PAGEREF _Toc467903144 \h 4 Business model and other relevant information PAGEREF _Toc467903145 \h 5 External Analysis PAGEREF _Toc467903146 \h 5 The PESTEL Analysis PAGEREF _Toc467903147 \h
S ECTOR /I NDUSTRY R ESEARCH W ORKSHEET S ECTOR /I NDUSTRY R ESEARCH W ORKSHEET Name: James Musasizi Period: 5 Team Name: Wolf Squad By grouping stocks into sectors and industries, it is easier for investors to evaluate stocks within the same industry and assess the economic strength or weakness of that industry. Go to a stock research website, such as Google Finance or Yahoo! Finance, and find one stock in an industry under each sector listed below. Provide the stock name, ticker symbol, current stock price, and products/services of
Coronary Artery Disease Coronary Artery Disease Student's Name University affiliation Course Instructor Date Coronary Artery Disease Coronary Artery Disease (CAD) is the most common of the known heart diseases. This disease develops when the arteries that supply the heart with blood, nutrients and oxygen are damaged. Coronary Artery Disease is characterized with hardened and narrowed blood vessels as result of the buildup of cholesterol and other materials known as plague in the arterial walls (Wessel, 2004). Plague a
Resolution Paper UNICEF Resolution Paper UNICEF Topic : Combating Child Labour in Developing Countries Sponsors: Pakistan , USA, Brazil Signatories: Democratic Republic of Congo, Zimbabwe, Portugal , Belgium, Afghanistan Committee: UNICEF Recalling the UN Declaration on Human Rights which states that all children should have the right to free compulsory education for at least the elementary level of education , Bearing in mind the continual worldwide atroci ty that child labour presents in today's economic society, Ob
Dhruv Patel Dhruv Patel 300856248 Scrapbook Week 2: Meditation and relaxation I often have meditated in the past because I had free time. However, with my school work, my social life, and other needs, I have taken away my time to relax. After this build up, I've been stressing out over little things that should not have a big impact on to my life. For example, I was on the bus and on my way home. I was tired and hungry, and it was extremely hot that day. I wanted to sit because my muscles were trembling af
Social Determinants of Health Connection with High Social Determinants of Health Connection with High Costs High Users of The Canadian Health Care System Fatima Zahid 213465976 HLST 2020: Power and Politics Kimberley Sauder March 20th 2017 It has been acknowledged for many years that a small number of individuals have been high users of the Canadian healthcare system taking up a large percentage of health care costs. This essay argues; social determinants of health is chronically linked to creating high users and high costs of the health care s
Legalizing MarijuanaLegalizing Marijuana To the AIDS or cancer patient, it is the plant that fights nausea and appetite loss. To the nutritionist, its seed is second only to the soybean in nutritional value, and is a source of cooking oil and vitamins. To the paper or cloth manufacturer, it is the plant that provided much of our paper and clothing for hundreds of years and produces four times more fiber per acre than trees. To the environmentalist, it is the plant that could greatly slow deforestation, restore robb
BulimiaBulimia Bulimia is an eating disorder marked by consumption of large quantities of food at one sitting, followed by induced vomiting of taking laxatives to get rid of the body food. It usually affects young women, especially adolescents, but is observed in older women and men also. People with bulimia are often obsessed with food and self gratification, and the disorder frequently accompanies Anorexia Nervosa. Such behavior stems from psychological difficulties involving a compulsive desire for
Tobacco And Western CultureTobacco and Western Culture Grade Received on Report : 89 Essay 1: Tobacco and Western Culture The use of tobacco dates back to the 17th century. The primary reason for its beginning was purely economic at first. It later became apparent to researchers over the decades that tobacco was more than just a commodity to be traded for economic gain. It was actually a drug, nicotine, which developed into physical dependency and had adverse side effects as people began to live longer. Further research a
Nutritional FactsNutritional Facts The first nutritional fact most Americans learn is that iron builds strong and healthy bodies.1 The beef lobby, cereal manufacturers, bread makers, and drug companies have bombarded the public with iron being the cure-all for fatigue and iron-poor blood. People have been mislead by drug companies pushing iron supplements and by old-fashioned ideas about iron, the magical nutrient of strength. Even the cartoons of the past pushed iron as the secret ingredient in Popeye's spi
The Ice AgeThe Ice Age Twenty thousand years ago during the time of the last ice age period, many colossal mammals roamed North America. They survived during the times when much of the earth was covered by immense large bodies of ice that buried forests, fields, and mountains, but rapidly became extinct after the ice began to retreat and melt. Since then the human race has introduced many different theories to explain the extinction of these large mammals. One theory stands above all and explains the truth
Female ReproductionFemale Reproduction Female Reproduction Lesson 1 • ovary - a gland that produces estrogen and ova. Ova are female reproductive cells. Ova also are called eggs. An ovum is one egg. • fallopian tube - a four inch long tube through which ova move from an ovary to the uterus. A female has two Fallopian tubes; one near each ovary. • uterus - an organ that supports a fertilized egg during pregnancy. The uterus is muscular and stretches when a baby is growing inside. • cervix - the lower part of the ut
Spent Mushroom WasteSpent Mushroom Waste as a Media Replacement for Peat Moss in Kai-Lan (Brassica oleracea var. Alboglabra) Production , Spent mushroom waste (SMW), otherwise known as spent mushroom substrate (SMS) or spent mushroom compost (SMC), is the leftover after different flushes of mushrooms have been harvested . Normally, at the end of each production cycle, “spent” (used) mushroom substrates are left abandoned or discarded. One of the major environmental problems in the mushroom producing countries re
Operating Systems and Software All Wrapped Up Operating Systems and Software All Wrapped Up April Page, Ian Keane, Nathan Cook, Alma Molina, Luis Wiley and Christine Lehman BIS 320 Business Information Systems July 27, 2015 Klöckner Pentaplast Europe GmbH & Co. KG produces films for packaging, printing, and specialty solutions to pharmaceutical, medical device, food, beverage, and card markets among others worldwide. Klöckner is a Global based company in Canada, Germany, Switzerland, Ohio, Virginia, Thailand, Mexico, Russia Argentina, Braz
Nutritional Competency Howard University College of Dentistry Dental Hygiene Department Nutritional Competency Comprehensive Dental Periodontology Nicole Patrice Johnson Professor Sandra Osborne 11/23/2015 Patient’s Nutritional Daily Log (3 Days 5/17/15 – 5/19/15) FOOD DIARY NAME_ Veronica Strong-Davis________ TEL_______123-123-3234____________ AGE_____57________SEX_____F____ Date: 5/17/2015 Type of Foods/Beverage Quantity Eaten (cup, oz, tbsp.,tsp,etc) Preparation Method BREAKFAST Toast 2 Slices Toasted Eggs 2 Eggs
LABOUR LAW PROJECT 3 LABOUR LAW PROJECT ON MATERNITY BENEFIT ACT SUBMITTED BY: STUTI PRIYA (00516503813) R.SHRAVYA (09116503813) B.A.LL.B. 8TH SEMESTER INTRODUCTION In the ancient time, the employer thought that maternity was a state of disability for woman workers from doing any work during the few weeks immediately before and after child birth. The employer tended that this period is wastage of both time and money, so simply they terminated the services of woman workers when they inferred any maternity situatio
What Are Some Natural Diuretics? What Are Some Natural Diuretics? By Nadine Ali, eHow Contributor Diuretics are foods or drugs that help eliminate water retention by increasing urination. They are often prescribed to help ease bloating during PMS and in the treatment of kidney, liver and heart conditions as well as for weight loss. Natural diuretics in the form of fruits, vegetables and herbs are safer and preferable to synthetic products. Other People Are Reading Natural Skincare Treatments What Are the Benefits of Natural Cl
VegetarianismVegetarianism Vegetarianism is the limitation of ones diet to only vegetables. Vegetarianism has been around for centuries, but recently we have seen the eruption of a more militant vegetarianism that is inspired by the animal rights movement. Today, vegetarian activists are throwing pies at Ronald McDonald and the Pork Queen, scrawling meat is murder in prominent locations, committing terrorist acts of arson, and waging media campaigns equating meat consumption with cannibalism. Vegetarians
Objectification and Humanization Objectification and Humanization People in almost every developed country and culture on earth have attained unprecedented levels of personal freedom and equality to their fellow human beings. Of course, people did not suddenly start enjoying this relative freedom and equality at the turn of the 21st century. It took centuries of social movements, hundreds of poignant social thinkers, and a countless number of social activists to get from a social structure of nobility and peasants to the equal